JOVE JOURNAL

Biochemistry

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Journal (Biochemistry)

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Gabrielle R. Budziszewski¹, M. Elizabeth Snell¹, Tiffany R. Wright¹, Miranda L. Lynch¹, Sarah E. J. Bowman^1,2

¹National High-Throughput Crystallization Center,Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences,The State University of New York

Journal (Biochemistry)

Oil and Crystallization Cocktail Well Plate Preparation

Gabrielle R. Budziszewski¹, M. Elizabeth Snell¹, Tiffany R. Wright¹, Miranda L. Lynch¹, Sarah E. J. Bowman^1,2

¹National High-Throughput Crystallization Center,Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences,The State University of New York

Journal (Biochemistry)

Protein Sample Setup and Crystallization

Gabrielle R. Budziszewski¹, M. Elizabeth Snell¹, Tiffany R. Wright¹, Miranda L. Lynch¹, Sarah E. J. Bowman^1,2

¹National High-Throughput Crystallization Center,Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences,The State University of New York

Journal (Biochemistry)

Image Analysis to Identify Protein Crystals

Gabrielle R. Budziszewski¹, M. Elizabeth Snell¹, Tiffany R. Wright¹, Miranda L. Lynch¹, Sarah E. J. Bowman^1,2

¹National High-Throughput Crystallization Center,Hauptman-Woodward Medical Research Institute, ²Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences,The State University of New York

Journal (Biochemistry)

High-Throughput Protein Crystallization via Microdialysis

Tristan O. C. Kwan¹, Amy E. Danson¹, Pascelle Draper², Paul Reardon², Isabel Moraes¹

¹National Physical Laboratory, ²SWISSCI LTD

Journal (Biochemistry)

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Jitske Bak¹, Lisa Landskron¹, Thijn R. Brummelkamp¹, Anastassis Perrakis¹

¹Oncode Institute and Division of Biochemistry,Netherlands Cancer Institute

Journal (Biochemistry)

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Núria Crua Asensio¹, Stefano Rizzieri², Alessandro Cuomo², Gian Gaetano Tartaglia^1,3, Elsa Zacco¹

¹Center for Human Technology,Italian Institute of Technology (IIT), ²Department of Experimental Oncology,European Institute of Oncology (IEO), ³Department of Biology and Biotechnologies ‘Charles Darwin’,Sapienza University of Rome

Journal (Biochemistry)

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Shuo Wang¹, Stephan Nussberger¹

¹Biophysics Department, Institute of Biomaterials and Biomolecular Systems,University of Stuttgart

Journal (Biochemistry)

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Xinghui Song¹, Hao Shen², Yanwei Li³, Yueting Xing³, Jiajia Wang³, Chun Guo³, Yingying Huang³, Jin Chen²

¹Central Laboratory of Women’s Hospital,Zhejiang University School of Medicine, ²School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University, ³Core Facilities,Zhejiang University School of Medicine

Journal (Biochemistry)

Malachite Green Assay for the Discovery of Heat-Shock Protein 90 Inhibitors

Sayan Dutta Gupta¹, Dae-Geun Song¹, SueJung Lee¹, Jae Wook Lee¹, Jin-Soo Park¹, Chrisostomos Prodromou², Cheol-Ho Pan¹

¹Gangneung Institute of Natural Products,Korea Institute of Science and Technology (KIST), ²School of Life Sciences,University of Sussex

Journal (Biochemistry)

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Isadora da Silva Zanzarini¹, Gustavo Barbosa¹, Larissa de Oliveira Prado¹, Ingrid Fatima Zattoni¹, Gustavo Da Paz¹, Ademir Luiz do Prado^2,3, Waldemar Volanski², Marcos Dinís Lavarda³, Fabiane Gomes de Moraes Rego², Geraldo Picheth², Vivian Rotuno Moure¹, Glaucio Valdameri¹

¹Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, ²Graduate Program in Pharmaceutical Sciences,Federal University of Parana, ³Federal Institute of Parana

Journal (Biochemistry)

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography

Ewa Rejnowicz¹, Jack Wright¹, Margaret Veldman-Jones¹, Steven Harborne¹

¹Peak Proteins Ltd.

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