JOVE JOURNAL

Biochemistry

Showing 157 - 168 in 776 videos.

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Journal (Biochemistry)

HeLa Cell Fixation with Hoechst 33342 and Blocking

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Journal (Biochemistry)

Addition of Primary and Secondary Antibodies to the Transfected HeLa Cells to Monitor the mtDNA Distribution

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Journal (Biochemistry)

Quantitative Image Analysis to Measure the mtDNA and Gene Expression Levels in the Transfected HeLa Cells

Lukasz S. Borowski^1,2, Karolina Kasztelan^2,3, Jolanta Czerwinska-Kostrzewska^1,2, Roman J. Szczesny²

¹Faculty of Biology, Institute of Genetics and Biotechnology,University of Warsaw, ²Institute of Biochemistry and Biophysics,Polish Academy of Sciences, ³International Institute of Molecular and Cell Biology in Warsaw

Journal (Biochemistry)

Cell Culture and Drug Treatment in the Human Embryonic Kidney 293 (HEK293) Cell Line

¹Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Journal (Biochemistry)

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

¹Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Journal (Biochemistry)

Isolation and Normalization of DNA Containing Crosslinked Proteins

¹Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Journal (Biochemistry)

Western Blotting of Digested DNA Samples Containing DNA-Protein Crosslinks (DPCs)

¹Developmental Therapeutics Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health

Journal (Biochemistry)

“Cell Surface Capture” Workflow for Label-Free Quantification of the Cell Surface Proteome

Akul Naik¹, Sanjeeva Srivastava^1,2, Arun P. Wiita^1,3,4

¹Department of Laboratory Medicine,University of California, San Francisco, ²Department of Biosciences and Bioengineering,Indian Institute of Technology Bombay, ³Deptartment of Bioengineering and Therapeutic Sciences,University of California, San Francisco, ⁴Chan Zuckerberg Biohub

Journal (Biochemistry)

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Jing Tong¹, Stefanie Rufli¹, Wendy Wei-Lynn Wong¹

¹Institute of Experimental Immunology,University of Zürich

Journal (Biochemistry)

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Alan Tin Lun Lam¹, Premkumar Jayaraman², Abigail Becker³, Ryan Lim², Kim Leng Teo¹, Jacqueline Ng², Steve Oh¹

¹Bioprocessing Technology Institute (BTI),Agency for Science, Technology and Research (A STAR), ²Thermo Fisher Scientific, ³Thermo Fisher Scientific

Journal (Biochemistry)

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Kyle Lethcoe¹, Colin A. Fox¹, Isabel Moh¹, Matthew Swackhamer¹, Michael Karo¹, Ryan Lockhart¹, Robert O. Ryan¹

¹Department of Biochemistry and Molecular Biology,University of Nevada

Journal (Biochemistry)

Immunostaining-Based Detection of Dynamic Alterations in Red Blood Cell Proteins

Marijke Grau¹, Lennart Kuck²

¹Department of Molecular and Cellular Sports Medicine,German Sport University Cologne, ²Biorheology Research Laboratory, Menzies Health Institute,Griffith University Gold Coast

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