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Titration of Human Coronaviruses Using an Immunoperoxidase Assay
章
- 00:11Titering Coronaviruses
- 00:54Introduction
- 01:36Purification of Virus from Tissue
- 03:43Purification of Virus from Cultured Cells
- 05:31Preparation of suseptible cells
- 08:20Virus Detection with the Immunoperoxidase Assay
- 11:26Conclusion
In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.
