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Advanced NMR Techniques for Glycan-Protein Interaction Analysis
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Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition
JoVE Journal
化学
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JoVE Journal 化学
Disentangling Glycan-Protein Interactions: Nuclear Magnetic Resonance (NMR) to the Rescue

Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Advanced NMR Techniques for Glycan-Protein Interaction Analysis

DOI:
10.3791/66530-v

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01:11 min

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May 17, 2024

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, , , ,
1CIC bioGUNE, Basque Research and Technology Alliance (BRTA), 2lkerbasque, Basque Foundation for Science, 3Centro de Investigacion Biomedica En Red de Enfermedades Respiratorias

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Glycan-protein InteractionsNuclear Magnetic Resonance (NMR)Saturation Transfer Difference (STD)Heteronuclear Single Quantum Coherence (HSQC)Molecular RecognitionChemical Glycobiology

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Author Spotlight: Unveiling the Structural and Dynamic Aspects of Glycan Molecular Recognition

Acquisition, Processing, and Analysis of 1H-Saturation Transfer Difference (STD) NMR to Characterize Protein-Glycan Interactions

15N-Heteronuclear Single Quantum Coherence (HSQC) Titration Experiments to Monitor the Chemical Shifts of the 1H and 15N NMR Resonances

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