ジャーナル
/
発生生物学
/
Diferenciação e Caracterização de Osteoclastos de Células-Tronco Pluripotentes Induzidas Humanas
JoVE Journal
発生生物学
Author Produced
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Diferenciação e Caracterização de Osteoclastos de Células-Tronco Pluripotentes Induzidas Humanas

1,191 Views

•

10:52 min

•

March 22, 2024

DOI:

10.3791/66527-v

DOI:
10.3791/66527-v

•

10:52 min
March 22, 2024

•

68 Views
, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

概要

Automatically generated

Este protocolo apresenta a diferenciação de osteoclastos humanos de células-tronco pluripotentes induzidas (iPSCs) e descreve métodos para a caracterização de osteoclastos e precursores de osteoclastos.

Explore More Videos

OsteoclastsHuman Induced Pluripotent Stem Cells (iPSCs)DifferentiationCharacterizationPassagingCD34+ Peripheral Blood Mononuclear CellsBone Disease Researchがん研究Endoprosthesis ResearchExpandabilityDMEM/F-12DispaseHEPESCell CultureCell Aggregates

Read Article

Cite this Article

Blümke, A., Simon, J., Leber, E., Scatena, M., Giachelli, C. M. Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (205), e66527, doi:10.3791/66527 (2024).

Download .ris file

Copy

Share Video

.

Copy