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Locus-Specific Chromatin Isolation from Yeast Cells

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Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

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JoVE Journal 生化学

Single-Copy Gene Locus Chromatin Purification in Saccharomyces cerevisiae

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Locus-Specific Chromatin Isolation from Yeast Cells

DOI:

10.3791/65460-v

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01:51 min

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November 17, 2023

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Atiqa Sajid, Stephan Hamperl

¹Institute of Epigenetics and Stem Cells,Helmholtz Zentrum München

タグ

Chromatin Purification Single-copy Gene Locus Saccharomyces Cerevisiae Eukaryotic Nucleus Genomic Instability Protein-DNA Interactions Nucleosome Core Particle Chromatin Remodeling Affinity Purification Mass Spectrometry

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Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Yeast Cell Culture and Cell Harvesting for Single-Copy Gene Locus Chromatin Purification

Locus-Specific Chromatin Purification from Saccharomyces cerevisiae

TEV Protease-Mediated Elution and Denaturation Elution of the Chromatin Locus Purified from Saccharomyces cerevisiae

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