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Improved Protein Quantification Using Two-Step Mitochondrial Isolation

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Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

JoVE Journal
生物学

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JoVE Journal 生物学

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Author Spotlight: Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

Improved Protein Quantification Using Two-Step Mitochondrial Isolation

DOI:

10.3791/65252-v

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01:45 min

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June 02, 2023

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Joan Blanco-Fernandez, Alexis A. Jourdain

¹Department of Immunobiology,University of Lausanne

タグ

Mitochondrial Isolation Two-step Purification Differential Centrifugation Immune Capture Mass Spectrometry Proteomics Metabolomics CRISPR-Cas9 Mitochondrial Function Energy Metabolism Immune Response Cancer Metabolic Disorders Anti-inflammatory Effects Mitochondrial Proteome

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