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Kultivierung von Ferkel-Darm-3D-Organoiden aus kryokonservierten Epithelkrypten und Etablierung von Zellmonoschichten
JoVE Journal
生物学
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JoVE Journal 生物学
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
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Kultivierung von Ferkel-Darm-3D-Organoiden aus kryokonservierten Epithelkrypten und Etablierung von Zellmonoschichten

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08:19 min

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February 10, 2023

DOI:

10.3791/64917-v

DOI:
10.3791/64917-v

•

08:19 min
February 10, 2023

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6 Views
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1GenPhySE,Université de Toulouse, INRAE, ENVT, 2Lallemand Animal Nutrition, 3Institut NuMeCan, INRAE, INSERM,Université de Rennes, 4FG 16: Mycotic and Parasitic Agents and Mycobacteria,Robert Koch-Institute

タグ

Pig Intestinal EpitheliumIntestinal Organoids3D OrganoidsCryopreserved CryptsEpithelial Crypts2D MonolayersNutrient TransportBarrier FunctionHost-microbe InteractionsJejunumDuodenumIleumColonExtracellular MatrixECMCulture MediumPassagingDissociationDMEMRock Inhibitor

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Mussard, E., Lencina, C., Boudry, G., Achard, C. S., Klotz, C., Combes, S., Beaumont, M. Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers. J. Vis. Exp. (192), e64917, doi:10.3791/64917 (2023).

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