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Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone
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JoVE Journal 発生生物学
Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

DOI:
10.3791/64794-v

•

07:36 min

•

March 17, 2023

•

, , ,
1Department of Plant and Soil Sciences,University of Delaware

章

  • 00:04Introduction
  • 00:40Infecting Barley (Hordeum vulgare) with Rice Blast Fungus (Magnaporthe oryzae)
  • 03:11Staining the Infected Leaf Sheaths
  • 04:12Mounting and Imaging of the Stained Leaf Sheaths
  • 05:51Results: Infected and Stained Sheaths Imaging
  • 06:57Conclusion

概要

自動翻訳

自動翻訳

This is a straightforward protocol of a barley leaf sheath assay using minimal reagents and common laboratory equipment (including a basic smartphone). The purpose is to visualize the early infection process of blast disease in labs without access to advanced microscopy equipment.

タグ

BarleyRice Blast DiseaseMagnaporthe OryzaeInfection SitesVisualizationMicroscopySmartphoneSheathConidial SuspensionHumiditySpore Germination

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