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Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
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JoVE Journal 免疫学と感染
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA

DOI:
10.3791/64644-v

•

07:18 min

•

May 12, 2023

•

, , , ,
1Department of Biochemistry and Biotechnology,University of Science and Technology Chittagong (USTC), 2Department of Emergency and Critical Care Medicine,Aichi Medical University

章

  • 00:04Introduction
  • 01:18Sample Collection and Storage
  • 02:13Modified Sandwich Enzyme‐Linked Immunosorbent Assay
  • 05:42Results: Myeloperoxidase‐DNA (MPO‐DNA) and Neutrophil Elastase‐DNA (NE‐DNA) Complex Quantification
  • 06:54Conclusion

概要

自動翻訳

自動翻訳

We present a protocol for a modified sandwich enzyme-linked immunosorbent assay technique to quantitatively measure two components of neutrophil extracellular trap remnants, myeloperoxidase conjugated-DNA and neutrophil elastase conjugated-DNA complexes,derived from activated neutrophils.

タグ

Myeloperoxidase-DNANeutrophil Elastase-DNANETsELISASepsisARDSDamage-associated Molecular PatternsPolymorphonuclear NeutrophilsPMADNase IEDTA

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