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使用 ImageJ 自动分析 沙门氏菌 的细胞内表型
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
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使用 ImageJ 自动分析 沙门氏菌 的细胞内表型

2,390 Views

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10:39 min

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August 09, 2022

DOI:

10.3791/64263-v

DOI:
10.3791/64263-v

•

10:39 min
August 09, 2022

•

8 Views
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1Risk Analysis and Genomic Epidemiology Unit,Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER)

概要

Automatically generated

沙门氏菌在沙门氏菌特异性液泡中和细胞质中游离（超复制）侵入并复制肠上皮细胞内。这里描述了一种基于高通量荧光显微镜的方案，通过 ImageJ 进行两次互补图像分析来量化沙门氏菌的细胞内表型，达到单细胞分辨率和评分。

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Cite this Article

Berni, M., Pongolini, S., Tambassi, M. Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ. J. Vis. Exp. (186), e64263, doi:10.3791/64263 (2022).

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