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Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ

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10:39 min

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August 09, 2022

DOI:

10.3791/64263-v

DOI:
10.3791/64263-v

•

10:39 min
August 09, 2022

•

8 Views
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1Risk Analysis and Genomic Epidemiology Unit,Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER)

Summary

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Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

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Cite this Article

Berni, M., Pongolini, S., Tambassi, M. Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ. J. Vis. Exp. (186), e64263, doi:10.3791/64263 (2022).

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