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Deep Fluorescence Observation in Rice Shoots via Clearing Technology
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生物学
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JoVE Journal 生物学
Deep Fluorescence Observation in Rice Shoots via Clearing Technology

Deep Fluorescence Observation in Rice Shoots via Clearing Technology

2,416 Views

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07:21 min

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June 27, 2022

DOI:

10.3791/64116-v

DOI:
10.3791/64116-v

•

07:21 min
June 27, 2022

•

5 Views
, , ,
1Graduate School of Bioagricultural Sciences,Nagoya University, 2Bioscience and Biotechnology Center,Nagoya University, 3Institute for Advanced Research (IAR),Nagoya University, 4Institute of Transformative Bio-Molecules (ITbM),Nagoya University

概要

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The present protocol describes a clearing technique for rice shoots, which are difficult to prepare for internal structural observations owing to the hard, thick, or layered nature of the tissues. This method facilitates continuous and deep fluorescence observations, even in adult rice plants.

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Cite this Article

Niimi, Y., Nagai, K., Ashikari, M., Mizuta, Y. Deep Fluorescence Observation in Rice Shoots via Clearing Technology. J. Vis. Exp. (184), e64116, doi:10.3791/64116 (2022).

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