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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
JoVE Journal
神経科学
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JoVE Journal 神経科学
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

1,749 Views

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08:26 min

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August 23, 2022

DOI:

10.3791/64111-v

DOI:
10.3791/64111-v

•

08:26 min
August 23, 2022

•

6 Views
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1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine,The University of Tokyo

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Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

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Simultaneous ImagingMicroglia DynamicsNeuronal ActivityAwake MiceAAV InjectionCranial WindowStereotaxic InstrumentMotion ArtifactPrimary Visual CortexMicroinjector

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Cite this Article

Maruoka, H., Kamei, R., Mizutani, S., Liu, Q., Okabe, S. Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice. J. Vis. Exp. (186), e64111, doi:10.3791/64111 (2022).

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