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Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
JoVE Journal
生物学
This content is Free Access.
JoVE Journal 生物学
Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications

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09:00 min

June 10, 2022

DOI:

09:00 min
June 10, 2022

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概要

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This protocol details a method for the dissection of mouse adipose depots and the isolation and digestion of respective arteries to liberate and then identify the endothelial cell population. Freshly isolated cells used in downstream applications will advance the understanding of vascular cell biology and the mechanisms of vascular dysfunction.

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