This article presents a protocol for the formation of microtubule assemblies in the shape of tactoids using MAP65, a plant-based microtubule crosslinker, and PEG as a crowding agent.
Chauhan, P., Sahu, S., Goodbee, N., Martin, S., Lee, H. B., Branch, R., Schwarz, J. M., Ross, J. L. Self-Assembly of Microtubule Tactoids. J. Vis. Exp. (184), e63952, doi:10.3791/63952 (2022).