We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.
Sevak, J. K., Gopinath, S. D. Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage. J. Vis. Exp. (186), e63725, doi:10.3791/63725 (2022).