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10:55 min
March 21, 2022
DOI:
10.3791/63602-v
该协议描述了一种以p53肿瘤抑制蛋白为例从哺乳动物细胞中纯化泛素蛋白的分步方法。泛素化的p53蛋白在严格的非变性和变性条件下从细胞中纯化。
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Wu, D., He, M., Li, D. Purification of Ubiquitinated p53 Proteins from Mammalian Cells. J. Vis. Exp. (181), e63602, doi:10.3791/63602 (2022).
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