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Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System
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December 10, 2021
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We provide scalable protocols covering construct design, transient transfection, and expression and purification of full-length human huntingtin protein variants in HEK293 cells.
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Pace, J. B., Huang, N. N., Séguin, J. P., Esquina, C., Olin, E., Zhu, G., Carr, G. Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System. J. Vis. Exp. (178), e63190, doi:10.3791/63190 (2021).
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