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Pyrosequencing: A Simple Method for Accurate Genotyping
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January 08, 2008
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Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.
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PyrosequencingGenotypingPharmacogenetic ResearchHuman Genome ProjectDataGenotyping MethodsPolymorphismsTri-allelicIndelsShort-repeat PolymorphismsAllele PercentagesMethylationPooled Sample AssessmentInternal Quality ControlSingle-nucleotide Polymorphism EvaluationClinically Relevant PolymorphismsTechniqueMethodology
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King, C., Scott-Horton, T. Pyrosequencing: A Simple Method for Accurate Genotyping. J. Vis. Exp. (11), e630, doi:10.3791/630 (2008).
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