The combined use of microelectrode array technology and 4-aminopyridine-induced chemical stimulation for investigating network-level nociceptive activity in the spinal cord dorsal horn is outlined.
Iredale, J. A., Stoddard, J. G., Drury, H. R., Browne, T. J., Elton, A., Madden, J. F., Callister, R. J., Welsh, J. S., Graham, B. A. Recording Network Activity in Spinal Nociceptive Circuits Using Microelectrode Arrays. J. Vis. Exp. (180), e62920, doi:10.3791/62920 (2022).