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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles
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生物学
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Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles

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09:55 min

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August 12, 2021

DOI:

10.3791/62843-v

DOI:
10.3791/62843-v

•

09:55 min
August 12, 2021

•

13 Views
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1Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution,Johannes Gutenberg University, 2Department of Insect Symbiosis,Max Planck Institute for Chemical Ecology, 3Department of Plant and Environmental Sciences, Section for Organismal Biology,University of Copenhagen

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This is an adapted method for identifying candidate insect colonization factors in a Burkholderia beneficial symbiont. The beetle host is infected with a random mutant library generated via transposon mutagenesis, and library complexity after colonization is compared to a control grown in vitro.

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Transposon Insertion SequencingBurkholderia GladioliLagria Villosa BeetlesBacterial ColonizationGenetic ManipulationSymbiotic BacteriaConditionally Essential GenesE. Coli DonorBurkholderia Gladioli RecipientConjugationMutant Library

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Cite this Article

Ganesan, R., Kaltenpoth, M., Flórez, L. V. Transposon-insertion Sequencing as a Tool to Elucidate Bacterial Colonization Factors in a Burkholderia gladioli Symbiont of Lagria villosa Beetles. J. Vis. Exp. (174), e62843, doi:10.3791/62843 (2021).

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