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Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence
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July 05, 2021
DOI:
1Vanderbilt Eye Institute,Vanderbilt University Medical Center, 2Department of Cell and Developmental Biology,Vanderbilt University, 3Department of Biochemistry,Vanderbilt University, 4Division of Clinical Pharmacology, Department of Medicine,Vanderbilt University Medical Center, 5Division of Hematology and Oncology,Indiana University School of Medicine, 6Department of Molecular Physiology and Biophysics,Vanderbilt University
概要
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This article provides a detailed methodology for the measurement of isolevuglandins in tissues by immunofluorescence using alkaline phosphatase-conjugated ScFv D11 antibody. Hypertension models in both mice and humans are used to explain the step-by-step procedures and fundamental principles associated with isolevuglandin measurement in tissue samples.
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Warden, C., Simmons, A. J., Pasic, L., Pitzer, A., Davies, S. S., Layer, J. H., Mernaugh, R. L., Kirabo, A. Direct Detection of Isolevuglandins in Tissues Using a D11 scFv-Alkaline Phosphatase Fusion Protein and Immunofluorescence. J. Vis. Exp. (173), e62603, doi:10.3791/62603 (2021).
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