ジャーナル
/
生化学
/
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
JoVE Journal
生化学
Author Produced
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

DOI:
10.3791/62589-v

•

12:26 min

•

February 12, 2022

•

, ,
1Helmholtz Institute for RNA-based Infection Research (HIRI),Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), 2Medical Faculty,Julius-Maximilians University Würzburg

章

  • 00:02Introduction
  • 01:56Sample Preparation
  • 04:20Instrument Setup
  • 05:47Sample Measurement
  • 09:19Data Analysis
  • 10:06Representative Results
  • 12:18Conclusion

概要

自動翻訳

自動翻訳

This protocol presents a complete experimental workflow for studying RNA-protein interactions using optical tweezers. Several possible experimental setups are outlined including the combination of optical tweezers with confocal microscopy.

タグ

Optical TweezersRNA-protein InteractionsTranslation RegulationSingle Molecule TechniquesFluorescence Assisted Optical TweezersForce-ramp ExperimentsConstant Force MeasurementsReal-time VisualizationDNA-RNA ConstructIn-vitro TranscriptionPCR Reactions

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Quantitative Immunofluorescence to Measure Global Localized Translation

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Read Article