Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Taylor R. Jamieson; Joanna Poutou; Ricardo Marius; Xiaohong He; Reza Rezaei; Taha Azad; Carolina S. Ilkow

doi:10.3791/62486

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免疫学と感染

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Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

JoVE Journal
免疫学と感染

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JoVE Journal 免疫学と感染

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

4,052 Views

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10:25 min

•

June 05, 2021

DOI:

10.3791/62486-v

DOI:

10.3791/62486-v

•

10:25 min

June 05, 2021

•

9 Views

Taylor R. Jamieson^1,2, Joanna Poutou^1,2, Ricardo Marius^1,2, Xiaohong He¹, Reza Rezaei^1,2, Taha Azad^1,2, Carolina S. Ilkow^1,2

¹Ottawa Hospital Research Institute, ²Department of Biochemistry, Microbiology and Immunology,University of Ottawa

概要

The protocol described here outlines a fast and effective method for measuring neutralizing antibodies against the SARS-CoV-2 spike protein by evaluating the ability of convalescent serum samples to inhibit infection by an enhanced green fluorescent protein-labeled vesicular stomatitis virus pseudotyped with spike glycoprotein.

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Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

概要

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