We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.
Yu, W., Ishan, M., Wang, Z., Liu, H. Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice. J. Vis. Exp. (167), e62163, doi:10.3791/62163 (2021).