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Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
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生化学
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JoVE Journal 生化学
Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

6,433 Views

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06:18 min

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April 23, 2021

DOI:

10.3791/62079-v

DOI:
10.3791/62079-v

•

06:18 min
April 23, 2021

•

24 Views
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1Department of Nephrology, Medical Faculty,Heinrich-Heine-University

概要

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The presented method enables visualization of fluorescently labeled cellular proteins with expansion microscopy leading to a resolution of 70 nm on a conventional microscope.

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Cite this Article

Königshausen, E., Schmitz, C. T., Rump, L. C., Sellin, L. Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy. J. Vis. Exp. (170), e62079, doi:10.3791/62079 (2021).

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