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Preparing Adult Drosophila melanogaster for Whole Brain Imaging during Behavior and Stimuli Responses
章
- 00:05Introduction
- 00:31Head Slot Creation
- 01:44Fly Placement
- 02:39Securing the Head
- 03:36Body Positioning and Hole Sealing
- 04:54Head Dissection
- 05:59Results: Representative Drosophila melanogaster Whole Brain Imaging and Analysis
- 07:14Conclusion
We present a method specifically tailored to image the whole brain of adult Drosophila during behavior and in response to stimuli. The head is positioned to allow optical access to the whole brain, while the fly can move its legs and the antennae, the tip of the proboscis, and the eyes can receive sensory stimuli.
