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低温样品加载到魔法角度旋转核磁共振光谱仪中，可保持细胞生存能力
JoVE Journal
生物学
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JoVE Journal 生物学
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
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低温样品加载到魔法角度旋转核磁共振光谱仪中，可保持细胞生存能力

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06:42 min

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September 01, 2020

DOI:

10.3791/61733-v

DOI:
10.3791/61733-v

•

06:42 min
September 01, 2020

•

2 Views
, , ,
1Department of Biophysics,University of Texas Southwestern Medical Center, 2Center for Alzheimer’s and Neurodegenerative Disease and Center for Systems Biology,University of Texas Southwestern Medical Center

概要

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这里介绍了冷冻样品低温传输到动态核极化 （DNP） 神奇角度旋转 （MAS） 核磁共振 （NMR） 探测器的协议。该协议包括实验前转子存储方向和实验前后可行性测量方向。

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Cite this Article

Ghosh, R., Kragelj, J., Xiao, Y., Frederick, K. K. Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability. J. Vis. Exp. (163), e61733, doi:10.3791/61733 (2020).

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