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Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
Chapters
- 00:04Introduction
- 00:49Cryopreservation of Mammalian Cells in an NMR Rotor
- 01:25Cryogenic Transfer of a Frozen Sample into the NMR Spectrometer
- 03:48Cryogenic Removal of the Sample from the NMR Spectrometer
- 05:02Results: Cryogenic Insertion of a Pre-frozen Sample of Mammalian Cells Results in Cellular Intactness Similar to Never-frozen Mammalian Cell Samples
- 05:47Conclusion
Presented here is a protocol for cryogenic transfer of frozen samples into the dynamic nuclear polarization (DNP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) probe. The protocol includes directions for rotor storage prior to the experiment and directions for viability measurements before and after the experiment.
