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Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

3,097 Views

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06:42 min

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September 01, 2020

DOI:

10.3791/61733-v

DOI:
10.3791/61733-v

•

06:42 min
September 01, 2020

•

2 Views
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1Department of Biophysics,University of Texas Southwestern Medical Center, 2Center for Alzheimer’s and Neurodegenerative Disease and Center for Systems Biology,University of Texas Southwestern Medical Center

Summary

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Presented here is a protocol for cryogenic transfer of frozen samples into the dynamic nuclear polarization (DNP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) probe. The protocol includes directions for rotor storage prior to the experiment and directions for viability measurements before and after the experiment.

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Cite this Article

Ghosh, R., Kragelj, J., Xiao, Y., Frederick, K. K. Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability. J. Vis. Exp. (163), e61733, doi:10.3791/61733 (2020).

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