ジャーナル
/
免疫学と感染
/
High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

DOI:
10.3791/61597-v

•

07:25 min

•

February 23, 2021

•

,
1Department of Chemical and Biomolecular Engineering,University of Houston

章

  • 00:05Introduction
  • 00:33Microarray Plate Screening
  • 03:26Validating the Identified Conditions
  • 05:30Results: Microarray and Validation
  • 06:36Conclusion

概要

自動翻訳

自動翻訳

In this method paper, we present a high-throughput screening strategy to identify chemical compounds, such as osmolytes, that have a significant impact on bacterial persistence.

タグ

High-throughput ScreeningBacterial PersistenceChemical CompoundsDrug PanelsGene LibrariesMicroarrayOfloxacinPersister AssayColony Forming Units (CFUs)Serial DilutionOrbital ShakerModified LB Medium

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.

A High-throughput Shigella-specific Bactericidal Assay

Automated, High-Throughput Detection of Bacterial Adherence to Host Cells

Read Article