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Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations
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Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations

4,641 Views

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08:51 min

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May 05, 2021

DOI:

10.3791/61453-v

DOI:
10.3791/61453-v

•

08:51 min
May 05, 2021

•

22 Views
, , , ,
1遺伝学科,University of Georgia, 2生化学・分子生物学科,University of Georgia, 3Complex Carbohydrate Research Center,University of Georgia, 4Department of Molecular Biosciences,Northwestern University

概要

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To use Caenorhabditis elegans (C. elegans) in omics research, a method is needed to generate large populations of worms where a single sample can be measured across platforms for comparative analyses. Here, a method to culture C. elegans populations on large-scale culture plates (LSCPs) and to document population growth is presented.

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Cite this Article

Shaver, A. O., Gouveia, G. J., Kirby, P. S., Andersen, E. C., Edison, A. S. Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations. J. Vis. Exp. (171), e61453, doi:10.3791/61453 (2021).

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