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使用无缝基因组编辑将点突变引入人类多能干细胞
JoVE Journal
遺伝学
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JoVE Journal 遺伝学
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
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使用无缝基因组编辑将点突变引入人类多能干细胞

3,636 Views

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09:03 min

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May 10, 2020

DOI:

10.3791/61152-v

DOI:
10.3791/61152-v

•

09:03 min
May 10, 2020

•

5 Views
, ,
1MRC再生医学センター,University of Edinburgh

概要

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在这里，我们描述了一种使用基于 piggyBac的供体质粒和Cas9切口酶突变体在人类多能干细胞中进行无缝基因编辑的详细方法。将两点突变引入人胚胎干细胞（hESCs）肝细胞核因子4α（HNF4α）位点的外显子8。

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Cite this Article

Wang, Y., Smith, A. J. H., Hay, D. C. Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing. J. Vis. Exp. (159), e61152, doi:10.3791/61152 (2020).

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