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Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
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JoVE Journal 免疫学と感染
Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis

2,823 Views

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09:02 min

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February 20, 2021

DOI:

10.3791/61133-v

DOI:
10.3791/61133-v

•

09:02 min
February 20, 2021

•

9 Views
, , , ,
1Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d’Intervention Biologique d’Urgence (CIBU), 3Cellule Pasteur,Université de Paris

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Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions.

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Cite this Article

Labadie, T., Grassin, Q., Batéjat, C., Manuguerra, J., Leclercq, I. Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis. J. Vis. Exp. (168), e61133, doi:10.3791/61133 (2021).

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