Measuring heart rate during a thermal challenge provides insight into physiological responses of organisms as a consequence of acute environmental change. Using the American lobster (Homarus americanus) as a model organism, this protocol describes the use of impedance pneumography as a relatively noninvasive and nonlethal approach to measure heart rate in late stage invertebrates.
Harrington, A. M., Haverkamp, H., Hamlin, H. J. Impedance Pneumography for Minimally Invasive Measurement of Heart Rate in Late Stage Invertebrates. J. Vis. Exp. (158), e61096, doi:10.3791/61096 (2020).