ジャーナル
/
生化学
/
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

9,415 Views

•

08:31 min

•

April 10, 2020

DOI:

10.3791/61016-v

DOI:
10.3791/61016-v

•

08:31 min
April 10, 2020

•

15 Views
, , ,
1生化学・分子生物学科,McGovern Medical School at UT Health, 2MD Anderson UT Health Graduate School

概要

Automatically generated

Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples.

Explore More Videos

Acyl-resin Assisted CaptureAcyl-RACProtein S-acylationThioester Bond CleavageChloroform-methanol PrecipitationProtein PelletCell LysisProtein Concentration EstimationBradford AssayBCA Assay

Read Article

Cite this Article

Tewari, R., West, S. J., Shayahati, B., Akimzhanov, A. M. Detection of Protein S-Acylation using Acyl-Resin Assisted Capture. J. Vis. Exp. (158), e61016, doi:10.3791/61016 (2020).

Download .ris file

Copy

Share Video

.

Copy