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In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
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JoVE Journal 発生生物学
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

DOI:
10.3791/60982-v

•

08:56 min

•

May 05, 2020

•

, ,
1Division of Biological Sciences,University of Montana

章

  • 00:04Introduction
  • 00:49Dissection/Gonad Extrusion
  • 02:46Fixation/Blocking
  • 04:14Primary Antibody Incubation and PLA Probe (Secondary Antibody) Incubation
  • 05:21Ligation
  • 06:09Amplification and Final Washes
  • 06:45Coverslip Mounting
  • 07:15Results: Germlines Following Co-immunostaining or PLA
  • 08:10Conclusion

概要

自動翻訳

自動翻訳

This protocol demonstrates use of the proximity ligation assay to probe for protein-protein interactions in situ in the C. elegans germline.

タグ

Proximity Ligation AssayProtein-protein InteractionsC. ElegansGermlineDissectionImmunofluorescenceMicroscopy

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