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Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes
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JoVE Journal 免疫学と感染
Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

4,151 Views

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08:52 min

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February 05, 2021

DOI:

10.3791/60949-v

DOI:
10.3791/60949-v

•

08:52 min
February 05, 2021

•

2 Views
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1Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, 2Stony Brook Cancer Center, Stony Brook, NY, 3医学部,Weill Cornell Medicine, New York, NY

概要

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Here, we provide a detailed description of the procedure to induce colonoscopic-guided pinch biopsies in mice and track wound closure in real time. Additionally, methods for the preparation of tissues for histological, immunohistochemical and molecular analyses of the wound bed are provided.

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Cite this Article

Montrose, D. C., McNally, E. M., Sue, E., Dannenberg, A. J. Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes. J. Vis. Exp. (168), e60949, doi:10.3791/60949 (2021).

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