This protocol presents a detailed procedure to prepare biological cryosamples for synchrotron-based X-ray absorption spectroscopy experiments. We describe all the steps required to optimize sample preparation and cryopreservation with examples of the protocol with cancer and phytoplankton cells. This method provides a universal standard of sample cryo-preparation.
Bissardon, C., Isaure, M., Lesuisse, E., Rovezzi, M., Lahera, E., Proux, O., Bohic, S. Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy. J. Vis. Exp. (183), e60849, doi:10.3791/60849 (2022).