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High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
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生物学
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JoVE Journal 生物学
High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

7,065 Views

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08:18 min

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June 16, 2020

DOI:

10.3791/60800-v

DOI:
10.3791/60800-v

•

08:18 min
June 16, 2020

•

9 Views
, , , ,
1Advanced ICT Research Institute Kobe,National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences,Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry,University of Oxford

概要

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Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

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Cite this Article

Matsuda, A., Koujin, T., Schermelleh, L., Haraguchi, T., Hiraoka, Y. High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon. J. Vis. Exp. (160), e60800, doi:10.3791/60800 (2020).

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