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An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
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JoVE Journal 免疫学と感染
An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

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07:32 min

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December 03, 2020

DOI:

10.3791/60790-v

DOI:
10.3791/60790-v

•

07:32 min
December 03, 2020

•

8 Views
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1Medical Clinic 1, University of Erlangen-Nuremberg,University Hospital of Erlangen, 2Norwich Medical School,University of East Anglia, Norwich Research Park

概要

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Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.

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Cite this Article

Martínez-Sánchez, L. D., Pradhan, R., Ngo, P. A., Erkert, L., Becker, L. S., Watson, A. J., Atreya, I., Neurath, M. F., López-Posadas, R. An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance. J. Vis. Exp. (166), e60790, doi:10.3791/60790 (2020).

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