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Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography
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JoVE Journal 発生生物学
Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

5,434 Views

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08:22 min

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February 11, 2020

DOI:

10.3791/60766-v

DOI:
10.3791/60766-v

•

08:22 min
February 11, 2020

•

9 Views
1Department of Biochemistry and Molecular Genetics, Alcohol Research Center,University of Louisville

概要

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This work describes a protocol to quantify ethanol levels in a zebrafish embryo using head space gas chromatography from proper exposure methods to embryo processing and ethanol analysis.

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EthanolZebrafishEmbryosGas ChromatographyPharmacokineticsChorionProteaseVolume MeasurementEmbryo ProcessingTime-course

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Cite this Article

Lovely, C. B. Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography. J. Vis. Exp. (156), e60766, doi:10.3791/60766 (2020).

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