Discovering CsgD Regulatory Targets in <em>Salmonella</em> Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

Melissa  B. Palmer; Yejun Wang; Aaron P. White

doi:10.3791/60736

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Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

JoVE Journal
遺伝学
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JoVE Journal 遺伝学

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

7,200 Views

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13:48 min

•

January 18, 2020

DOI:

10.3791/60736-v

DOI:

10.3791/60736-v

•

13:48 min

January 18, 2020

•

8 Views

Melissa B. Palmer^1,2, Yejun Wang³, Aaron P. White^1,2

¹Vaccine and Infectious Disease Organization-International Vaccine Centre, ²Department of Biochemistry, Microbiology and Immunology,University of Saskatchewan, ³Department of Cell Biology and Genetics,Shenzhen University Health Science Center

概要

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a method used to establish interactions between transcription factors and the genomic sequences they control. This protocol outlines techniques for performing ChIP-seq with bacterial biofilms, using Salmonella enterica serovar Typhimurium bacterial biofilm as an example.

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ChIP-seq Chromatin Immunoprecipitation Transcription Factors DNA-binding Proteins Salmonella Biofilms CsgD Gene Regulation Bacterial Gene Expression High-throughput Sequencing Regulatory Targets

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Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)

概要

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