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Measurement of Natural Killer Cell-Mediated Cytotoxicity and Migration in the Context of Hepatic Tumor Cells
章
- 00:05Introduction
- 00:52Colorimetric Lactic Dehydrogenase (LDH) Measurement-Based Natural Killer (NK) Cell-Mediated Cytotoxicity Assay
- 02:46Calcein AM Staining-Based NK Cell-Mediated Cytotoxicity Assay
- 04:10NK Cell Migration Assay
- 05:26Results: Representative NK Cell-Mediated Cytoxicity and Migration
- 06:17Conclusion
Evasion of natural killer (NK) cell-mediated eradication by cancer cells is important for cancer initiation and progression. Here, we present two non-radioactivity-based protocols to evaluate NK cell-mediated cytotoxicity toward hepatic tumor cells. Additionally, a third protocol is presented to analyze NK cell migration.
