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Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
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Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

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07:19 min

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February 18, 2020

DOI:

10.3791/60704-v

DOI:
10.3791/60704-v

•

07:19 min
February 18, 2020

•

18 Views
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1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus,Technische Universität Dresden

概要

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This protocol describes an efficient electroporation method for the transfection of four different gastrointestinal organoid entities with larger plasmids (to the extent of 10 kB). It can be performed within one day and does not need extensive preparation or special, cost-intensive electroporation buffers.

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Cite this Article

Gaebler, A., Hennig, A., Buczolich, K., Weitz, J., Welsch, T., Stange, D. E., Pape, K. Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids. J. Vis. Exp. (156), e60704, doi:10.3791/60704 (2020).

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