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Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

15,874 Views

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07:19 min

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February 18, 2020

DOI:

10.3791/60704-v

DOI:
10.3791/60704-v

•

07:19 min
February 18, 2020

•

11 Views
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1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus,Technische Universität Dresden

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ElectroporationGenetic EngineeringOrganoids3D Cell CultureTransfectionUniversal ProtocolEfficientTumor OrganoidsHealthy OrganoidsSpheroids2D Cell CultureDissociationY-27632Basal MediumElectroporation BufferPlasmid DNAImpedanceElectroporation Parameters

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Gaebler, A., Hennig, A., Buczolich, K., Weitz, J., Welsch, T., Stange, D. E., Pape, K. Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids. J. Vis. Exp. (156), e60704, doi:10.3791/60704 (2020).

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