ジャーナル
/
生化学
/
硅氮化物膜的细胞培养和同步加速器X射线荧光纳米分析的冷冻制备
JoVE Journal
生化学
This content is Free Access.
JoVE Journal 生化学
Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
Please note that all translations are automatically generated. Click here for the English version.

硅氮化物膜的细胞培养和同步加速器X射线荧光纳米分析的冷冻制备

8,679 Views

•

08:26 min

•

December 10, 2019

DOI:

10.3791/60461-v

DOI:
10.3791/60461-v

•

08:26 min
December 10, 2019

•

36 Views
, , , , , ,
1Inserm, UA7, Synchrotron Radiation for Biomedicine (STROBE),Université Grenoble Alpes, 2ID16A Beamline,ESRF, The European Synchrotron

概要

Automatically generated

这里介绍的是一个协议，用于氮化硅膜上的细胞培养和在X射线荧光成像之前使用同步加速器低温X射线纳米探针进行跳投。当只提供室温纳米分析时，冷冻样品可以进一步冷冻干燥。这些是获得细胞内元素组成信息的关键步骤。

Explore More Videos

Cell CultureSilicon Nitride MembraneCryopreparationSynchrotron X-ray Fluorescence Nano-analysisCryofixationFrozen Hydrated CellsElemental DistributionRadiation DamageCell MonolayerPoly-L-lysineAttachment FactorUltra Pure WaterCell SeedingCell Culture Medium

Read Article

Cite this Article

Bissardon, C., Reymond, S., Salomé, M., André, L., Bayat, S., Cloetens, P., Bohic, S. Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis. J. Vis. Exp. (154), e60461, doi:10.3791/60461 (2019).

Download .ris file

Copy

Share Video

.

Copy