ジャーナル
/
がん研究
/
原发性慢性淋巴细胞白血病细胞的细胞次细胞分段监测核/细胞质蛋白贩运
JoVE Journal
がん研究
This content is Free Access.
JoVE Journal がん研究
Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
Please note that all translations are automatically generated. Click here for the English version.

原发性慢性淋巴细胞白血病细胞的细胞次细胞分段监测核/细胞质蛋白贩运

10,876 Views

•

11:39 min

•

October 23, 2019

DOI:

10.3791/60426-v

DOI:
10.3791/60426-v

•

11:39 min
October 23, 2019

•

3 Views
, , ,
1Paul O’Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences,University of Glasgow

概要

Automatically generated

该协议能够优化和随后有效地从原发性慢性淋巴细胞白血病细胞中生成核质和细胞质部分。这些样本用于确定蛋白质定位以及细胞刺激和药物治疗后核质室和细胞质室之间的蛋白质贩运变化。

Explore More Videos

Subcellular FractionationChronic Lymphocytic LeukemiaNuclear/cytoplasmic Protein TraffickingTumor Suppressor ProteinsPathogenesisB Cell MalignanciesProtein LocalizationProtein MovementDensity Gradient CentrifugationMononuclear Cells

Read Article

Cite this Article

Hay, J., Moles, M. W., Cassels, J., Michie, A. M. Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking. J. Vis. Exp. (152), e60426, doi:10.3791/60426 (2019).

Download .ris file

Copy

Share Video

.

Copy