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Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
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JoVE Journal 免疫学と感染
Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

6,543 Views

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09:43 min

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December 07, 2019

DOI:

10.3791/60397-v

DOI:
10.3791/60397-v

•

09:43 min
December 07, 2019

•

1 Views
, , , , , , ,
1Infections in Hematology and Oncology,Leibniz Institute for Infection Biology and Natural Product Research, 2Department for Hematology and Medical Oncology,Jena University Hospital, 3Institute for Transfusion Medicine,Jena University Hospital

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This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to leukocytes.

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PhagocytosisAspergillus FumigatusConidiaHuman LeukocytesFlow CytometryCell MarkersNeutrophilsMonocytesMucoralesRed Blood CellsFITC LabelingRPMI MediumBuffy CoatEL BufferCentrifugation

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Cite this Article

Hartung, S., Rauh, C., Böttcher, S., Hoang, M. T. N., Jahreis, S., Rummler, S., Hochhaus, A., von Lilienfeld-Toal, M. Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry. J. Vis. Exp. (154), e60397, doi:10.3791/60397 (2019).

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