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Generation of Multicellular Human Primary Endometrial Organoids
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JoVE Journal 発生生物学
Generation of Multicellular Human Primary Endometrial Organoids

Generation of Multicellular Human Primary Endometrial Organoids

10,544 Views

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09:20 min

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October 04, 2019

DOI:

10.3791/60384-v

DOI:
10.3791/60384-v

•

09:20 min
October 04, 2019

•

6 Views
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1Department of Obstetrics and Gynecology,Northwestern University, 2National Center for Genetic Engineering and Biotechnology (BIOTEC),National Science and Technology Development Agency (NSTDA)

概要

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A protocol to generate human primary endometrial organoids that consist of epithelial and stromal cells and retain characteristics of the native endometrial tissue is presented. This protocol describes methods from uterine tissue acquisition to the histologic processing of endometrial organoids.

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Murphy, A. R., Wiwatpanit, T., Lu, Z., Davaadelger, B., Kim, J. J. Generation of Multicellular Human Primary Endometrial Organoids. J. Vis. Exp. (152), e60384, doi:10.3791/60384 (2019).

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